Most human introns are recognized via multiple and tissue-specific branchpoints
نویسندگان
چکیده
منابع مشابه
Human and mouse introns are linked to the same processes and functions through each genome's most frequent non-conserved motifs
We identified the most frequent, variable-length DNA sequence motifs in the human and mouse genomes and sub-selected those with multiple recurrences in the intergenic and intronic regions and at least one additional exonic instance in the corresponding genome. We discovered that these motifs have virtually no overlap with intronic sequences that are conserved between human and mouse, and thus a...
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Multiple introns have been found in a gene from a ciliated protozoan. This Tetrahymena thermophila gene (cnjB) is large (7.5 kb mRNA) and active only during conjugation, the organism's sexual cycle. Six introns ranging in size from 62 bp to 676 bp were found when we sequenced a 3.1 kb segment of the cnjB gene together with its corresponding cDNA. We estimate, by extrapolation of our current dat...
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متن کاملUsing branchpointer for annotation of intronic human splicing branchpoints
The spliceosome mediates the formation of an intron lariat though inter-action between the 5’ splice site and branchpoint (Will and Luhrmann, 2011). A subsequent reaction at the 3’ SS then removes the intron lariat, producing a spliced RNA product. Mapping of branchpoints generally requires sequencing of the intron lariat following cDNA synthesis (Gao et al., 2008; Taggart et al., 2012). Howeve...
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The use of biomaterials for gene delivery can potentially avoid many of the safety concerns with viral gene delivery. However, the efficacy of polymeric gene delivery methods is low, particularly in vivo. One significant concern is that the interior and exterior composition of polymeric gene delivery nanoparticles are often coupled, with a single polymer backbone governing all functions from bi...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 2018
ISSN: 0890-9369,1549-5477
DOI: 10.1101/gad.312058.118